Yes there is good evidence that acetaminophen (the active ingredient in Tylenol) can deplete glutathione under certain conditions. But whether that matters depends on dose, duration, and individual factors. Top 7 FAQs On Liposomal Glutathione vs. Traditional Glutathione West Bengal Chemical Industries Limited Silymarin prevents acetaminophen induced hepatotoxicity via up regulation of the glutathione conjugation capacity in mice ScienceDirect Tylenol's Impact on Glutathione and Liver Damage Taking Tylenol? The Glutathione Connection Dr. Green Mom Myth busting, Acetaminophen version. "Avoid Acetaminophen (Tylenol) because it causes glutathione depletion". Truth?? As a pediatrician and ER doctor and as moms we're often asked about this. And we understand why: