Yes there is good evidence that acetaminophen (the active ingredient in Tylenol) can deplete glutathione under certain conditions. But whether that matters depends on dose, duration, and individual factors. The multiple mechanisms and modes of cell death after acetaminophen overdose Glutathione, Alcohol and drugs Acetaminophen Toxicity: How Genetic Susceptibility Combines with Environmental Factors g glutamyl cycle. Depletion of glutathione by acetaminophen leads to Download Scientific Diagram Acetaminophen, also known as Tylenol, is used to help with pain & to reduce fever. However, taking too much acetaminophen can be harmful to the liver which filters out toxins &